Extended Data Fig. 6: Delayed inhibition increases notochord progenitor cells.

a, Nucleus 3D quantification of high-resolution 24 h delay colonies (mean 4991 ± 907 cells per colony) showing the proportions of cell types obtained by immunofluorescence. 44 ± 8% of cells acquired a TBXT + FOXA2+ identity in this 24 h delay condition (compare without delay in Fig. 3d). b, Percentage of single cells co-expressing TBXT + FOXA2 + NOTO + CHRD+ in each of the different delayed inhibition conditions showing 0.15% of NotoP cells in the constant inhibition neuruloid are boosted to 28% (using this single-cell transcriptome signature) in the 24 h delay condition. c, micropatterned coverslip overview of 24 h delay condition across cell lines. Scale bar: 200 μm colony (c).