Extended Data Fig. 10: TNE-ZsGreen H9 reporter line. | Nature

Extended Data Fig. 10: TNE-ZsGreen H9 reporter line.

From: Timely TGFβ signalling inhibition induces notochord

Extended Data Fig. 10

a, Single z-plane HCR stains of day7 notoroids for NOTO, CHRD and TNE-ZsGreen. b, Length and uninterrupted GFP signal measures in 3D (Imaris) based on lightsheet microscopy images (n = 4). c, independent replicate of Fig. 5j showing the distribution of ZsGreen signal along the midline of trunk-organoids. In the two replicates we detect an uninterrupted high signal for 30 ± 18% (Fig. 5j, n = 96) and 43 ± 19% (Extended Data Fig. 10c, n = 96) of the estimated length. Scale bars: 50 μm (a).

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