Extended Data Fig. 5: SKIc perturbations in cancer.
From: Synthetic lethality of mRNA quality control complexes in cancer
(a) Stacked bar chart of the frequency of homozygous deletions of FOCAD (grey), TTC37 (teal), SKIV2L (fuchsia), AVEN (purple) across TCGA cohorts with prevalence greater >5%. (b) Frequency of FOCAD mutations occurring across TCGA cohorts with prevalence >5%. (c) Generation of a SKIV2L rescue model in the NCI-H1299 SKIV2L KO cell line; validation of SKIV2L and TTC37 expression. Data is a representative image of a single replicate of n = 2 independent biological experiments. (d) PELO knockout in the panel of SKIV2L KO, SKIV2L WT rescue or SKIV2L R483C rescue cells. Data is a representative image of a single replicate of n = 3 independent biological experiments. (e) Relative viability of SKIV2L KO (−), SKIV2L WT rescue or SKVI2L R483C rescue cell lines measured 7 days after sgB2M (black), sgPELO (blue), or sgRAN (red) transfection. Data shown as mean ± s.e.m., n = 3 biologically independent replicates, two-tailed Student’s t-test between sgB2M and sgPELO groups; SKIV2L KO, P = 2.8 × 10−4; SKIV2L WT, P = 0.32; R483C, P = 9.5 × 10−7.
