Extended Data Fig. 2: Additional measures in the body including cardiac, % fat, and V02 and VCO2 did not differ between experimental groups.
From: The maternal X chromosome affects cognition and brain ageing in female mice
a, Diagram of organs assessed in mice during the middle-age life stage. Diagram of the mouse, heart, bone and cells in a are respectively adapted from AnaitSmi/Shutterstock, Vectorfair/Shutterstock, Panda Vector/Shutterstock and Designua/Shutterstock (https://www.shutterstock.com/). b, Fasting blood glucose levels did not differ between Xm+Xp and Xm mice either at young (Xm+Xp mice: n = 20; Xm mice: n = 23; age = 4-8 months) or old (Xm+Xp mice: n = 7; Xm mice: n = 10; age =24-27 months) ages. c-e, Cardiac echo was used to measure systole and diastole, fractional shortening, and ejection fraction (age = 16-19 months). c, Left ventricular volume measurements in diastole and systole did not differ between Xm+Xp and Xm-only, middle-aged mice (Xm+Xp diastole: n = 9; Xm diastole: n = 17; Xm+Xp systole: n = 9; Xm systole: n = 17). d, Fractional shortening measurements did not differ between Xm+Xp and Xm-only, middle-aged mice (Xm+Xp mice: n = 10; Xm mice: n = 18). e, Ejection fraction did not differ between Xm+Xp and Xm middle-aged mice (Xm+Xp mice: n = 10; Xm mice: n = 18). f-h, DEXA-scan of the body (age = 14-17 months) to measure bone density, and tissue mass. f, Bone density did not differ between Xm+Xp and Xm, middle-aged mice (Xm+Xp mice: n = 10; Xm mice: n = 17). g, Lean tissue mass did not differ between Xm+Xp and Xm, middle-aged mice (Xm+Xp mice: n = 10; Xm mice: n = 18). h, Body fat percentage did not differ between Xm+Xp and Xm mice (Xm+Xp mice: n = 10; Xm mice: n = 16). i-k, CLAMS metabolic cages (age = 14-17 months) were used to measure metabolic parameters including respiratory exchange ratio (RER) and energy expenditure. i, Respiratory exchange ratio (RER) did not differ between Xm+Xp and Xm, middle-aged mice (Xm+Xp mice: n = 9-10; Xm mice: n = 17-18). j, Energy expenditure did not differ between Xm+Xp and Xm, middle-aged mice (Xm+Xp mice: n = 10; Xm mice: n = 17-18). k, VO2 consumption did not differ between the groups (Xm+Xp mice: n = 10; Xm mice: n = 17-18). Two-way mixed model ANOVA: genotype P > 0.05. Data represent means ± SEM.
