Extended Data Fig. 6: The SpyCatcher system can be used to conjugate proteins to the surface of S. epidermidis.
From: Discovery and engineering of the antibody response to a prominent skin commensal
a, Coomassie staining of purified superfolder GFP (sGFP)-SpyTag003. b, S. epidermidis LM087 was engineered to express either a fusion between Aap and SpyCatcher (Aap-sc, yellow) or a catalytically dead version of SpyCatcher (Aap-sc*, grey). Both strains were incubated with 0.1, 0.3 or 1 mg ml−1 of sGFP-SpyTag for 2, 5, 15 or 60 min and visualized by flow cytometry. c, Surface expression of the transgene Aap-sc or Aap-sc* using a HA tag present on the construct for two different colonies (col) each. d, Coomassie staining of purified tetanus toxin fragment C (TTFC)-SpyTag003. e,f, Surface expression of Aap-sc and Aap-TTFC transgene on the corresponding strains as visualized on a flow plot (e) or the mean fluorescence intensity for four different colonies (f). a,d, One representative gel of at least three independent experiments. Uncropped Coomassie gels can be found in Supplementary Fig. 3c,d.
