Extended Data Fig. 7: Quantification of the conjugation efficiency of TTFC to the Aap-sc strain.
From: Discovery and engineering of the antibody response to a prominent skin commensal
a, Gating strategy for bacterial flow cytometry analysis. b, Schematic of Aap-sc* and conjugated Aap-sc-TTFC used in this figure. Both strains contain a HA tag fused to the SpyCatcher ___domain and TTFC-SpyTag003 a FLAG tag. c, Show the conjugation efficiency to TTFC-SpyTag003 for Aap-sc and Aap-sc* as measured by flow cytometry. d, Representative quantification experiment: (left) quantification beads and (right) four different colonies of Aap-sc conjugated to TTFC-SpyTag003. e, Quantification of the number of TTFC-SpyTag003 conjugated per bacteria and total amount of antigen given to mice per colonization for 6 colonies pooled from two different experiments. f, Formula used to calculate the total quantity of TTFC given per colonization using the #TTFC/bacterium calculated in d, e.
