Extended Data Fig. 5: Endogenous SIV Gag- and Env-specific T cell responses in barcoded SIV challenge study animals.
From: Antibody prophylaxis may mask subclinical SIV infections in macaques
Intracellular cytokine staining was performed on PBMCs to assess T cell responses to (A, C) SIV Gag and (B, D) Env peptide pools. (A, B) Samples assessed were collected before antibody infusion (pre-immune) and 2, 6 and 16 weeks post the dominant virus infection (see Tables S1–3). Circles and squares represent individual animals. Dotted lines are equal to 0%. The overlaid boxes and middle line indicate the IQR and median, respectively, and the whiskers are the range. N = 4 (control group) or n = 6 (ITS102.03 and ITS103.1-treatment groups) animals. (C, D) For animals where a viral blip was observed (animal IDs shown below horizontal axis and day post-first virus challenge of relevant blip in parentheses where appropriate), PBMCs collected 2 weeks after the blip were assayed and are shown relative to individual’s pre-immune measurement. Solid, horizontal lines are equal to 0%. (A–D) CD4+ T cell Th1 responses (IFNγ, IL-2, or TNF), CD4+ T cell Th2 responses (IL-4 or IL-13), and CD8+ T cell responses (IFNγ, IL-2, or TNF) are shown from left to right, respectively. Responses are background-subtracted.
