Fig. 4: Killing of S. aureus by flucloxacillin.
From: Limited impact of Salmonella stress and persisters on antibiotic clearance
a, Snapshots of gfp-expressing S. aureus (inverted fluorescence) before, during and after a 2 h exposure to flucloxacillin (FLX) and switching to antibiotic-free brain–heart infusion (BHI) medium (Supplementary Video 4). Scale bar, 5 μm. b, Surviving fractions of S. aureus after 1 h (left), 2 h (middle) or 4 h (right) exposure to flucloxacillin followed by switching to BHI medium (937, 745 and 599 cells pooled from three independent experiments). A growing colony originating from a single cell is counted as one survivor. Summary data for individual replicates are shown in c. c, Surviving fractions of S. aureus after 1 h, 2 h or 4 h exposure to flucloxacillin and after switching to antibiotic-free BHI medium. Data from three independent experiments (619, 745 and 917 cells). Lines connect the geometric means. Two-way ANOVA for difference between survival at the end of exposure and colony-forming fractions. The arrow depicts the post-exposure loss of viability.
