Extended Data Fig. 6: Single-cell transcriptomic analysis of skin-infiltrating immune cells at the site of vaccination.
From: A neoantigen vaccine generates antitumour immunity in renal cell carcinoma
a, UMAP representation of scRNA-seq of skin-infiltrating myeloid cells (N = 9 patients) at the vaccination site (right thigh). b, Heatmap of marker gene expression for each myeloid population, which was utilized to assign identity to each cell cluster. c, Boxplots of the proportion of each myeloid cell population before and after vaccination (two-sided paired Wilcoxon test; n = 7 paired samples, as n = 2 patients had insufficient material at baseline for scRNA-seq; boxplot hinges represent 25th to 75th percentiles, central lines represent the medians, the whiskers extend to lowest and highest values no greater than 1.5× interquartile range away from the 25th and 75th percentiles, and the dots indicate outliers). DC1: conventional dendritic cell (type 1); DC2: conventional dendritic cell (type 2); pDC: plasmacytoid dendritic cell. LC: Langerhans cell. d, UMAP representation of scRNA-seq of skin-infiltrating lymphoid cells (N = 9 patients) at the vaccination site (right thigh). e, Heatmap of marker gene expression for each lymphoid population, which was utilized to assign identity to each cell cluster. f, Boxplots of the proportion of each lymphoid cell population before and after vaccination (two-sided paired Wilcoxon test; n = 7 paired samples, as n = 2 patients had insufficient material at baseline for scRNA-seq; boxplot hinges represent 25th to 75th percentiles, central lines represent the medians, the whiskers extend to lowest and highest values no greater than 1.5× interquartile range away from the 25th and 75th percentiles, and the dots indicate outliers). NK cell: natural killer cell.
