Extended Data Fig. 8: Rapid and durable expansion of T cell clonotypes following vaccination.

a, For each of the n = 9 patients with peripheral blood TCR sequencing data available, the dynamics of T cell clonotypes with inferred vaccine specificity is shown following vaccination. Vaccine-expanded clonotypes were defined as undetectable or at the lower limit of detection at baseline (week 0), expands by at least 10-fold after vaccination in all subsequent timepoints (including at least 3 unique molecular identifier sequencing reads at ≥2 timepoints), and is identified in the skin during the DTH assessment. (left), Each gray line represents one T cell clonotype, and the red line represents the sum of all clonotypes for an individual patient. (middle) Each light blue line represents a CD4 clonotype, dark blue lines represent CD8 clonotypes, and gray represents unresolved clonotypes. (right) The summation of all clonotypes (red), all CD4 clonotypes (light blue), or all CD8 clonotypes (dark blue) for each patient. b, The mean (and standard error of the mean) vaccine-expanded TCR clonotype frequency over time for all n = 9 patients (linear scale). c, The median (and interquartile range) of vaccine-expanded TCR clonotype frequences over time for all n = 9 patients (red), also shown for all CD4 clonotypes (light blue) and CD8 clonotypes (dark blue).

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