Fig. 2: In vivo activity of COAplEsr1 cells during distinct bouts of investigation in male AGGs.
From: A crucial role for the cortical amygdala in shaping social encounters
a, Experimental timeline. b, COAplEsr1 activity before and after the investigation of an odour. c, There was a main effect of cue (F(5,40) = 3.689, P = 0.0077), time (F(1,8) = 10.89, P = 0.0108) and a cue × time interaction (F(5,24) = 7.716, P = 0.0002). Post-hoc comparisons revealed an increase in COAplEsr1 activity during the investigation of soiled male (P < 0.0001) and female (P = 0.0169) bedding (n = 9 male bedding, n = 9 female bedding, n = 5 peanut, n = 5 juvenile, n = 9 fox urine, n = 9 object). NS, not significant. d–i, COAplEsr1 activity before and after social behaviour. d,e, There was a significant increase in activity during investigation on an attack day (trial × time interaction: F(1,5) = 44.52, P = 0.0011; d) compared with investigation bouts during a no-attack day (P = 0.0002, Tukey’s post-hoc test; e). f,g, There was a significant increase in COAplEsr1 activity during investigation bouts that preceded an attack (behaviour × time interaction: F(1,5) = 9.054, P = 0.0298; f) compared with those that occurred in isolation (P = 0.0063, Tukey’s post-hoc (n = 6); g). h,i, There was a significant difference in COAplEsr1 activity before the onset of attack when mice were engaged in investigation before the attack (behaviour × time interaction: F(1,6) = 8.662, P = 0.0258; h) compared with when they were not investigating (P = 0.0175, Tukey’s post-hoc; i). No difference was observed in COAplEsr1 activity during attack, regardless of whether it was preceded by a bout of investigation (P = 0.9989, Tukey’s post-hoc) (n = 7). *P < 0.05, **P < 0.01, ***P < 0.001. All data are mean ± s.e.m. Scale bar, 200 μm. Data from this figure were replicated in two cohorts. The illustrations in a were created with BioRender.
