Extended Data Fig. 7: Lorazepam promotes tumor growth in vivo but has no effect on proliferation of patient-derived DMG cells in monoculture.
From: GABAergic neuron-to-glioma synapses in diffuse midline gliomas
a. LZP treatment of patient-derived DMG cultures SU-DIPGVI and SU-DIPGXIII-FL had no effect on malignant cell proliferation in monoculture (n = 3 wells per group). One-way ANOVA. P = 0.42 and P = 0.62, respectively. b. In vivo bioluminescent imaging (IVIS) quantification of tumor burden in mice bearing luciferase-expressing SU-DIPG-VI xenografts in the pons, treated with vehicle control or LZP (8 mg kg−1) at 14 days following the start of treatment (vehicle, n = 6 mice; LZP, n = 5 mice p = 0.0006). Data are represented as change from baseline luminescence (photon flux). Right, representative IVIS images of xenografted mice treated with LZP or vehicle control. Increased H3K27M+ DMG tumor burden is evident in the LZP-treated mice. Two-tailed Student’s t-test. All data are mean ± s.e.m. ***P < 0.001.
