Extended Data Fig. 3: DMG infiltration of human hippocampus and immuno-electron micrographs of GABAergic neuron-to-glioma synapses in hippocampal DMG xenografts.
From: GABAergic neuron-to-glioma synapses in diffuse midline gliomas
a. DMG infiltration of human hippocampus. 10x magnification light microscopy image of primary patient tissue with H3K27M+ DMG cells (brown) in the CA1 region of the hippocampus. Hematoxylin counterstain. DMG infiltration of hippocampus is not uncommon, as this case of a 14-year-old female with H3K27M-mutated DMG, also called diffuse intrinsic pontine glioma (DIPG), illustrates. Patient sample taken at time of autopsy. Scale bar, 100 µm. b-c. Immuno-electron microscopy of SU-DIPG-VI (n = 39 synapses identified from 4 mice, b) and SU-DIPGXIII-FL (n = 97 synapses identified from 3 mice, c) cells with GFP-tagged GABRG2 xenografted into mouse hippocampus. Six examples of GABAergic neuron-to-glioma synapses are shown here, in addition to those shown in Fig. 2a. Scale bar, 100 nm. d. Secondary-only control for immuno-electron microscopy of SU-DIPGXIII-FL with GFP-tagged GABRG2 xenografted into mouse hippocampus to demonstrate low background immuno-gold labelling. Scale bar, 1000 nm. n = 7 biological replicates.
