Extended Data Fig. 5: Cl− transporter expression in patient-derived xenografts of H3K27M + DMG.
From: GABAergic neuron-to-glioma synapses in diffuse midline gliomas

a. Current-voltage relationship of GABA current in two patient-derived DMG xenograft models recorded with perforated patch. Reversal potential of GABA was −25.0 ± 3.7 mV in SU-DIPGVI cells (n = 6 cells from 5 mice), and −20.7 ± 4.9 mV in SU-DIPGXIII-FL cells (n = 5 cells from 3 mice). b. Single cell RNAseq analysis of SLC12A2 (NKCC1) in two DMG patient-derived xenografts (PDX). c,d. Single cell RNAseq analysis illustrating expression of SLC12A2 (NKCC1; c) and SLC12A5 (KCC2; d) in patient-derived DMG xenografts (grey; n = 335 cells, 4 mice)3 and primary human biopsies of H3K27M+ diffuse midline glioma (grey; n = 2,259 cells, 6 study participants), IDH-wild-type high-grade glioma (IDH WT GBM; blue; n = 599 cells, 3 participants), and IDH-mutant high-grade glioma (IDH-mut GBM; purple; n = 5,096 cells, 10 participants) malignant cells15,32. Two-tailed Wilcoxon Rank Sum test. All data are mean ± s.e.m. ns, not significant.