Extended Data Fig. 2: Histology and single unit quality. | Nature

Extended Data Fig. 2: Histology and single unit quality.

From: Convergent vocal representations in parrot and human forebrain motor networks

Extended Data Fig. 2

a, Histological validation of silicon probe placement in zebra finch RA. Left, sagittal slice illustrating probe position, marked by DiI applied to the probe shank (red). Right, expanded view of the boxed region highlighting RA (white arrows) on the right. b, Histological validation of silicon probe placement in budgerigar AAC. Left, brightfield image of a coronal slice showing the position of the silicon probe, marked by DiI applied to the probe shank (red). Right, expanded view of the boxed region indicating dorsal AAC (AACd) and ventral AAC (AACv) by white and yellow arrows, respectively. c, Example single-unit waveforms and autocorrelograms from budgerigar AAC recordings. Mean waveforms (red) are overlaid on single-trial waveform traces, which are represented as gray lines. In the autocorrelogram, orange vertical lines indicate the refractory period (−1 to 1 ms). d, e, Distributions of peak-to-peak amplitude (d) and the percentage of refractory period violations (e) across all AAC single units (n = 220 from 4 birds). Illustrations of the zebra finch in a are reproduced from ref. 60, Elsevier.

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