Extended Data Fig. 1: Solid phase assays for receptor binding specificities.
From: Receptor-binding specificity of a bovine influenza A virus
The influenza A viruses (IAV) A/bovine/OH/B240SU-432/2024 (H5N1), A/Vietnam/1203/2004 (H5N1) and A/CA/04/2009 (pdmH1N1) (64 HA units) were captured on a fetuin-coated plate and incubated with 3’-SLN-PAA-biotin (10 µg/mL), 6’-SLN-PAA-biotin (10 µg/mL) or PBS in the presence or absence of neuraminidase inhibitor (Oseltamivir carboxylate, 2 μM). Virus-bound biotinylated polymer was detected using horseradish peroxidase (HRP)-conjugated streptavidin. The absorbance (A450) values for each treatment (in replicate of three, n = 3) of all viruses were plotted using Prism 10 software (GraphPad Software, Inc.). The assay was performed in triplicate at least two times for each virus, bars (scatter dot plot) represent the mean ± SEM (n = 3) for each treatment.
