Extended Data Fig. 3: Increased expression of BMAL1 and HIF2A in the border zone at ZT8.
From: BMAL1–HIF2A heterodimer modulates circadian variations of myocardial injury
a, Representative trichrome staining of heart sections on day 1 post‐MI. Border and remote areas are identified with an arrow and a star, respectively. The dashed lines highlight the extension of the areas of ischaemic injury, scale bar, 1 mm. b and d, Representative immunostaining of HIF2A (red) (b), BMAL1 (red) (d), WGA (green), and nuclei (DAPI; blue) in the border zone and remote area of hearts from C57BL/6J mice subjected to myocardial IRI at ZT8 or ZT20, n = 4 mice/group/time point, scale bar, 20 μm. White arrows indicate HIF2A or BMAL1 within the nuclei and cytoplasm. c and e, Quantification of fluorescence intensity of HIF2A in (b) and BMAL1 in (d). Normalized to the protein levels in the remote areas at ZT8. Each quantification value dot represents the average value of three fields in one section. n = same as above. Data are mean ± s.e.m. Statistical analysis was performed using two-way ANOVA.
