Extended Data Fig. 4: Increased induction of AREG in cardiomyocytes in the border zone at ZT8.
From: BMAL1–HIF2A heterodimer modulates circadian variations of myocardial injury
a-b, a, Representative immunostaining of AREG (red), α-sarcomeric (cardiomyocyte marker; green), and nuclei (DAPI; blue) on day 1 post-MI in the border zone, infarct area, and remote area of hearts from C57BL/6J mice subjected to myocardial IRI at ZT8 or ZT20, scale bar, 25 μm. Arrows indicate α-sarcomeric+/AREG+ cells. b, Quantification of fluorescence intensity of AREG in (a). Normalized to the AREG levels in the remote areas at ZT8. Each quantification value dot represents the average value of three fields in one section. n = 4 mice/group/time point. Data are mean ± s.e.m. Statistical analysis was performed using two-way ANOVA. c-d, Representative immunostaining of AREG (red), vimentin (fibroblast marker; green) (c), α-smooth muscle actin (α-SMA, smooth muscle cell marker; green) (d) and nuclei (DAPI; blue) on day 1 post-MI in the border zone of hearts from C57BL/6J mice subjected to myocardial IRI at ZT8 or ZT20, scale bar, 25 μm. n = 4 mice/group/time point.
