Extended Data Fig. 10: AREG upregulates CD47 via STAT3 signalling to suppress MNP phagocytosis.
From: Radiation-induced amphiregulin drives tumour metastasis
a, Schematic of live cell imaging co-culture system with CellTracker Red tumour cells and CellTracker Green bone-marrow derived myeloid cells (BMDM) (top). Fraction of engulfed LLCAR+ and LLCAR− tumour cells after 4 h, n = 10 technical replicates (bottom). Created in BioRender, Piffkó A, 2025. https://biorender.com/t54k444. b, Live cell imaging of co-culture system with CellTracker labelled tumour cells and BMDMs. c, Cd47 expression in LLCAR+ (left) and LLCAR− post-RT (right), measured by RT-qPCR, n = 2 technical replicates. d, Histogram and quantification of CD47 mean fluorescence intensity (MFI) on LLCAR+ and LLCAR− cells ± 4 Gy, n = 2 technical repeats. e, MFI of p-STAT3 (Tyr705) in LLC cells, after 25 min of rAR treatment at indicated concentrations, n = 3 technical replicates. One-way ANOVA, Šídák’s test. f, Cd47 expression in LLCAR+ cells post-rAR treatment, measured by RT-qPCR, n = 2 technical replicates. g, Cd47 expression in LLCAR+ cells post-RT, rAR, and STAT3i (Stattic, 5 μM) treatment, measured by RT-qPCR, n = 2 technical replicates. h, IF staining of Ly6C+ MNPs co-cultured with LLC tumour cells, stained for MyoIIA (AF488, green) and CD47 (BV421, blue). Close-up of phagocytic synapse. i, IF staining of Ly6C+ MNPs co-cultured with LLCAR+ (left) and LLCAR− (right). Stained for p-MyoIIA (AF488, green), Ly6C (PE/Dazzle 594, red), and CD47 (BV421, blue). Data are shown as mean ± SEM. One representative experiment out of two independent experiments is shown.
