Extended Data Fig. 11: AREG blockade synergizes with anti-CD47 and increases MNP phagocytosis in vivo.
From: Radiation-induced amphiregulin drives tumour metastasis
a, Kaplan-Meier plot of progression-free survival stratified by AREG serum levels, measured by ELISA. n = 42 samples from n = 21 patients (pre-SBRT and after three cycles of ICB + SBRT). Log-rank (Mantel-Cox) test. b, Representative gating strategy for mouse lung tissue identifying RFP+ phagocytic MNP populations. c, Percentage of phagocytic RFP + Ly6C+ monocytes (left), RFP + Ly6G+ neutrophils, RFP + CD11c+ MHCII+ dendritic cells, and RFP+ CD11b- Siglec-F+ alveolar macrophages shown as percentage of the parent population. Cells isolated from lungs of LLCAR+ tumour-bearing mice that were treated with 20 Gy RT or a combination with anti-AREG- (αAR) and anti-CD47-antibody (αCD47). n = 5 in LLCAR+ and 20 Gy group, n = 6 in other groups. One-way ANOVA, Šídák’s test. Box plots show median (Q2), Q1–Q3, and whiskers (min–max).
