Extended Data Fig. 6: Characterization of deacetylases T7dA and T9dA.
From: Discovery of FoTO1 and Taxol genes enables biosynthesis of baccatin III
a, Conversion of baccatin VI (11-Bz) to 9-dihydro-13α-acetylbaccatin III (9DHAB, 13) by T7dA and T9dA in N. benthamiana leaves. T7dA and T9dA were heterologously expressed in N. benthamiana via Agrobacterium-mediated transient expression and baccatin VI (11-Bz) 20 μM was fed to the leaves three days after infiltration. b, Reconstitution of 9DHAB (13) biosynthetic pathway in N. benthamiana. EICs of leaves expressing the reconstituted pathway compared to the 9DHAB standard are shown. MS/MS fragmentations were generated using [M+Na]+ (m/z = 653.2576) as the precursor ion and fragmented with a collision energy of 30 eV. Regions between m/z 100 and 800 are shown.
