Extended Data Fig. 2: The effects of gene RNAi knockdown.
From: Decoding 4-vinylanisole biosynthesis and pivotal enzymes in locusts
(a) The mRNA levels of LOCMI16699, LOCMI02868, LOCMI17143, LOCMI17606, LOCMI16705, and LOCMI03758 after injection of respective dsRNA in the locust hind leg. n = 6 biological replicates. (b-e) The emission of 4 VA after RNAi knockdown of LOCMI17143 (b), LOCMI17606 (c), LOCMI16705 (d), and LOCMI03758 (e). n = 6 biological replicates. P values were determined by a two-tailed unpaired t-test. (f and g) In vitro enzymatic activity assays of 4VPMT1(f) and 4VPMT2 (g). n = 3 replicates Data are presented as mean ± s.e.m.
