Fig. 3: R9AP receptor function depends on its N-terminal residues and TMDs.
From: R9AP is a common receptor for EBV infection in epithelial cells and B cells
a,b, Protein levels and EBV infection efficiency of ectopically expressed wild-type (WT) and truncated R9AP constructs (a) or WT and multiple deletions (b). c–e, EBV was pretreated with indicated peptide or control peptide (Ctrl) and added to HNE1 cells (c), Raji cells (d), primary NPECs or primary B cells (e) and EBV infection efficiency was analysed by flow cytometry (c,d) or qPCR (e). ****P <  0.0001. f, His–gH/gL was assayed for binding to Ctrl peptide or R9AP19–30. n = 2 independent experiments. g–i, Humanized B-NDG mice were infected with EBV and treated with Ctrl peptide or R9AP19–30 and EBV DNA copy number in peripheral blood was determined by qPCR at the indicated times (g,h). i, Survival curves of mice. n = 5 mice in each group. Data are mean ± s.e.m. (h). j–l, Primary B cells, NPECs, Akata, Raji, HNE1, AGS and HEK-293T cells were pretreated with R9AP monoclonal antibody (5E9) or IgG and infected with EBV, and EBV infection efficiency was analysed by counting colony formation using B cell transformation assays (j), qPCR (k) or flow cytometery (l). Data are mean ± s.e.m. from 3 independent experiments (a–d), primary B cells from 3 donors (e) performed in triplicate, n = 9 total replicates (a,c–e) or n = 8 total replicates (b). Data are mean ± s.e.m. from 1 representative of 2 (j) or 3 (k,l) independent experiments (n = 6 or 3 biological replicates). One-way ANOVA with Tukey’s correction for multiple comparisons (a,b); two-way ANOVA with Sidak’s correction for multiple comparisons (c–e,j–l); unpaired two-tailed t-test (h) and log-rank test (i). ****P < 0.0001. NS, not significant.
