Extended Data Fig. 9: Reduction in ethylene signalling levels lead to increase in suberized cell formation at the wound site.
From: Plants monitor the integrity of their barrier by sensing gas diffusion
a, Cross sections of 21-day-old wild-type, ein2-1, or etr1-3 roots. b, The density of suberized cells at the wound site was quantified in wild-type, ein2-1, or etr1-3 roots 2, 4, and 6 days after 15-day-old roots were injured. Two-tailed Wilcoxon rank-sum test was used (ns; P ≥ 0.05, *; P < 0.05). The difference between ein2-1 and etr1-3 would be due to the nature of mutations. Because etr1-3 is a dominant negative mutation, tissues in etr1-3 mutant still respond to ethylene when ETR1 is not expressed. c,d,f,g, Cross sections of 18-day-old proPER15:erVenus (c,f) or proPBP1:erVenus (d,g) roots at 1 dai grown without (Mock) or with 10 µM AVG (AVG) (c,d) or 0.5 mM AgNO3 (AgNO3) (f,g) for one day after the injury. The proportion of cells at the wound site showing Venus signal intensities above the threshold was quantified at 1 dai in mock, AVG or AgNO3-treated 18-day-old proPER15:erVenus and proPBP1:erVenus roots. Two-tailed Wilcoxon rank-sum test was used (ns; P ≥ 0.05, *; P < 0.05). e,h Cross sections of 21-day-old wild-type roots at 6 dai grown without (Mock) or with 10 µM AVG (AVG) (e) or 0.5 mM AgNO3 (AgNO3) (h) for six days after the injury. The density of suberized cells at the wound site was quantified in mock, AVG or AgNO3-treated 21-day-old wild-type at 6 dai. Two-tailed Wilcoxon rank-sum test was used (**; P < 0.01). n indicates the number of examined cross sections. Venus signal intensities in c,d,f,g and intensity of suberin staining with Fluorol yellow in a,e,h are shown according to the colour map on the right. White, SR2200 (cell wall). Scale bars: 50 µm.
