Extended Data Fig. 5: Wound sealing maintains high ethylene signalling and inhibits periderm regeneration.
From: Plants monitor the integrity of their barrier by sensing gas diffusion
a,b, Cross sections of 18-day-old RPS5A:erVenus (a) and RPS5A:erVenus-EBF1UTR (b) roots at 1 dai grown without (Cut) or with (Cut + Vaseline) Vaseline at the wound for one day after the injury. Roots sealed with Vaseline showed reduced erVenus-EBF1UTR signal than unsealed roots (b), thus indicating higher ethylene signalling in sealed roots. No changes were observed in RPS5A promoter activity (a). Venus signal intensities in distal phloem parenchyma near the wound were measured and normalized (c). Two-tailed Wilcoxon rank-sum test was used (ns; P ≥ 0.05, **; P < 0.01). c,d, Cross sections of 18-day-old proPER49:erVenus (c) and proPBP1:erVenus (d) roots at 1 dai grown without (Cut) or with (Cut + Lanolin) lanolin at the wound for one day after the injury. Proportion of cells at the wound site showing Venus signal intensities above a threshold (see methods) was quantified in 18-day-old proPER49:erVenus and proPBP1:erVenus seedlings at 1 dai. The wound was not (Cut) or was (Cut + Lanolin) covered with lanolin right after the injury. Two-tailed Wilcoxon rank-sum test was used (ns; P ≥ 0.05, **; P < 0.01). e, Cross sections of 21-day-old wild-type roots at 4 dai. The wound was not (Cut) or was (Cut + Lanolin) covered with lanolin for four days after the injury. The proportion of cross sections showing failure in suberized cell layer formation at the wound site in 21-day-old wild-type grown without (Cut) or with (Cut + Lanolin) lanolin at 4 dai. Two-sided Fisher’s exact test was used to test significant difference (**; P < 0.01) f, Cross sections of 18-day-old proPER15:erVenus roots at 1 dai grown in ambient air (Control) or under submergence (Submerged) for one day after the injury. The proportion of cells at the wound site showing Venus signal intensities above the threshold was quantified in 18-day-old proPER15:erVenus seedlings grown without (Control) or with (Submerged) submergence treatment for one day at 1 dai. Two-tailed Wilcoxon rank-sum test was used (**; P < 0.01). n indicates the number of examined cross sections. Venus signal intensities in a–d, f and intensity of suberin staining with Fluorol yellow in e are shown according to the colour map on the right. White, SR2200 (cell wall). Scale bars: 50 µm.
