Supplementary Figure 2: Myeloid and dendritic cell subclusters of the thymic hematopoietic cells observed with single-cell RNA-seq.
From: Inferring population dynamics from single-cell RNA-sequencing time series data
Here, we sub-clustered cluster 14_myeloid from Supplementary Fig. 2a into three clusters, again with louvain clustering. We show the expression of a dendritic and a myeloid cell marker across the t-SNE based on all thymic hematopoietic cells (n = 10895) observed with single-cell RNA-seq (a, b) to to show the overall restriction of these markers to the cluster 14_myeloid and across the three sub-clusters (c, d) to show the heterogeneity in this cluster. The violin plots in (c, d) are based on kernel density estimates and on n = 130 cells in group 0, n = 118 in group 1 and n = 102 in group 2. (a, c) Dendritic cell marker Cd74 log expression superimposed on t-SNE (a) and by louvain sub-cluster (c). (b, d) Myeloid cell marker Lyz2 log expression superimposed on t-SNE (b) and by louvain sub-cluster (d). See also Supplementary data 1.2 for the further details on this analysis.
