Supplementary Figure 10: Carbon flux distribution of S. coelicolor by metabolic flux analysis.

(a) Simplified metabolic network of S. coelicolor. Flux for biomass biosynthesis from the G6P node (v2) including fluxes from five different precursors (glucose-6-phosphate, fructose-6-phosphate, ribose-5-phosphate, erythrose-4-phosphate, glyceraldehyde-3-phosphate). 15 fatty acid moieties from cellular TAGs are detected in S. coelicolor, here flux v19 represents the sum of specific production rate of AcCoA from degradation of these fatty acids. The biochemical reactions, stoichiometric equations and experimentally determined fluxes are shown in Supplementary Table 6–8. (b) Actual fluxes of M145, HY01 and M145-DT in fermentation with (+) or without (-) glucose feeding. Data for metabolic flux analysis were collected during stationary phase (72–120 h). Data shown here were the average of three independent experiments. Abbreviations: Glc, glucose; G6P, glucose-6-phosphate; 3PG, 3-phosphate-glycerate; PEP, phosphoenolpyruvate; PYR, pyruvate; AcCoA, acetyl coenzyme A; AKG, alpha-ketoglutaric acid; OAA, oxaloacetic acid; TAG, triacylglycerol; Act, actinorhodin.