Supplementary Figure 11: Sampling analysis of flux distribution in M145 and HY01.
From: Harnessing the intracellular triacylglycerols for titer improvement of polyketides in Streptomyces
The genome-scale metabolic model (GEM) of S. coelicolor (iKS1317) (Biotechnol. J 14, e1800180, 2019) was constrained by the experimentally measured rate (Supplementary Table 8) with the minimization of glucose uptake rate as the objective function. During the simulation, the sampling algorithm optGpSampler (PLoS One 9, e86587, 2014) was adopted. For each condition, 1000 samples were used for valid sampling distributions. Here we showed two reactions from TCA cycle as examples. (a) Flux changes of reactions catalyzed by citrate synthase (CS) in batch fermentation (without glucose feeding). (b) Flux changes of reactions catalyzed by CS in fed-batch fermentation (with glucose feeding). (c) Flux changes of reactions catalyzed by aconitase (ACONTa) in batch fermentation (without glucose feeding). (d) Flux changes of reactions catalyzed by ACONTa in fed-batch fermentation (with glucose feeding).
