Extended Data Fig. 10: ISGS-identified impact scores at four chromatin remodeler genes in both T-ALL cells and T cells. | Nature Biotechnology

Extended Data Fig. 10: ISGS-identified impact scores at four chromatin remodeler genes in both T-ALL cells and T cells.

From: Cell-type-specific prediction of 3D chromatin organization enables high-throughput in silico genetic screening

Extended Data Fig. 10

a, Scatter plots showing impact scores of samples of screened regions (n = 10000). The impact score difference between target cell type (CUTLL1 and Jurkat) and T cell are shown on the x axis, and the higher impact scores between the corresponding cell type and the T cells are shown on y axis. b–e, Impact scores of the DNA elements in T-ALL cells and normal T cells were first calculated independently through ISGS and then visualized at the four chromatin remodeler genes (PHF5A (b), BRD4 (c), KAT5 (d) and CHD4 (e), with 50Kb upstream and 50Kb downstream) which are required for Jurkat and CUTLL1 cell proliferation according to the CRISPR screening experiments. The specificity track (fourth track) was calculated as the difference between T cell impact score and T-ALL impact score (from CUTLL1 or Jurkat, whichever is smaller). CHD4 has the highest specificity score between T-ALL cells and normal T cells.

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