Extended Data Fig. 3: Prime editing in Trp53-proficient cells and off-target activity at computationally predicted loci.
From: A prime editor mouse to model a broad spectrum of somatic mutations in vivo
a Prime editing in Trp53flox/flox;Rosa26PE2/+ versus Trp53+/+;Rosa26PE2/+ TTFs across several indicated loci. Data and error bars indicate the mean and standard deviation of three independent transductions. b Prime editing in Trp53flox/flox;Rosa26PE2/+ versus Trp53+/+;Rosa26PE2/+ pancreatic across several indicated loci. Note that the editing data for Dnmt1+GGG in Trp53flox/flox organoids are the same depicted in Fig. 2f. Data and error bars indicate the mean and standard deviation of three independent transductions. c Quantification of off-target prime editing for a pegRNA targeted to Dnmt1. Off-targets represent three computationally predicted loci for tail-tip fibroblasts cultured for more than one month in the presence of the Dnmt1+GGG pegRNA or a control pegRNA. Data and error bars indicate the mean and standard deviation of three independent transductions. Off-target locus details are described in Supplementary Table 4. d Quantification of off-target prime editing at four computationally predicted loci for tail-tip fibroblasts cultured with a KrasG12A epegRNA or a control pegRNA. Data and error bars indicate the mean and standard deviation of three independent transductions.
