Extended Data Fig. 9: Fluorescence imaging of bulk sialic acid on the cell surface of THP-1 monocyte, resting M0 macrophage (M0), LPS activated M0 macrophage (M0 + LPS).
From: Spatial imaging of glycoRNA in single cells with ARPLA
(a) Representative images of total sialic acid. These cells were metabolically labeled by Ac4ManNAz for 24 h, followed by incubation with DBCO-PEG4-biotin and Cy5-streptavidin for fluorescence imaging. Scale bars for cell image: 50 μm. (b) Quantification of the mean fluorescent intensity of the images, n = 3 biological replicates. Data are mean ±S.D. Unpaired two-tailed Student’s t-test determines the statistical significance. (**) p = 0.0013, (*) p = 0.0469.
