Extended Data Fig. 4: iBE induces low off target RNA editing that is reversed by withdrawal of transgene expression.
From: Generation of precision preclinical cancer models using regulated in vivo base editing
a. C to U editing in RNA transcripts detected from RNA sequencing data from intestine and liver from rtTAhem and iBEhom on normal chow (-), dox chow (+) for 14 days, or switched from dox chow for 14 days to normal chow for 6 days (SW). Data in the middle and right panels was derived from re-analysis of published datasets, as indicated under each plot. For experiments with multiple comparisons, p-values were calculated by one-way ANOVA, n=3 mice/condition. For individual pairwise comparisons, Student’s t-test was used. b. A to G editing in RNA transcripts detected from RNA sequencing data from intestine and liver from rtTAhem and iBEhom on normal chow (-), dox chow (+) for 14 days, or switched from dox chow for 14 days to normal chow for 6 days (SW). (n=3 mice). c. Transcript abundance (transcripts per million; TPM) in pancreatic organoids, intestine, and liver from rtTAhem and iBEhom on normal chow (-), dox chow (+) for 14 days, or switched from dox chow for 14 days to normal chow for 6 days (SW). All data shown are presented as mean values +/- s.d., n=3 mice/condition.
