Extended Data Fig. 10: Additional analyses of the Aurora dyes.

(a) Distribution of dyes among the hit and sensitive dyes assigned for each protein. Individual histograms displaying the number of hit dyes for a given protein, binned by the global hit rates of the specific hit dyes. Total hit rates are divided into ten evenly spaced bins, containing hit rates of up to 10, 20, 30, 40, 50, 60, 70, 80, 90, or 100% of all tested proteins. Green bars: for ‘hit’ assignment. Grey bars: for ‘sensitive’ assignment. The presence of both selective and promiscuous dye hits in an individual dye screen appears as the presence of hits in both lower hit rate (for example < 20%) and higher hit rate (for example >40%) bins. Results for individual proteins are presented in individual panels, with the protein name indicated in the panel title. (b) Weak positive correlation between protein sequence similarity and dye fingerprint. Comparison of the relationship between primary sequence similarity and dye screen outcomes shows a weak positive Pearson’s correlation: 0.19, p-value 3.9e-20. Statistical significance was determined by one-way ANOVA with Tukey’s post-hoc analysis. Data shown includes 2,211 unique pairings between 67 proteins (72 proteins tested total - 5 proteins tested only as hetero-complexes; see Supplementary Methods). To provide context, the number of dyes screened in common between two proteins is indicated in point size and color. Smaller, darker points: fewer dyes screened in common. Larger, lighter points: more dyes screened in common. See figure key for exact mappings. Calculations of the Jaccard Index (y axis values) and pairwise sequence similarity (x axis values) are described in Supplementary Methods.