Extended Data Fig. 2: Apparent melting temperatures for paDSF dyes and SYPRO Orange.
From: Protein-adaptive differential scanning fluorimetry using conformationally responsive dyes
Plots include data for the 60 protein test set, as well as the tested 10 SARS-CoV2 proteins. (a) For the subset of tested proteins compatible with SYPRO Orange, comparison of Tmas reported by Aurora library dyes and SYPRO Orange. Tmas between SYPRO Orange and Aurora dyes for the same protein are strongly correlated, with a Pearson R value of 0.97, p = 2.2 e-16. Statistical significance was determined by one-way t-test. Data shown includes 2,211 unique pairings(b) Tmas reported by all hit dyes from the Aurora library for all tested proteins. Green points = Mean Tma between all hit Aurora dyes for a given protein. Error bars represent ± standard error of Tmas between different Aurora dyes. Experiments were performed in triplicate. For proteins compatible with SYPRO Orange, Tma reported by SYPRO Orange is displayed as orange ‘X’s. Grey points: Tma of minor unfolding transitions, defined as secondary or tertiary transitions with lower RFU in a given unfolding curve.
