Extended Data Fig. 6: Independent analysis of 12-gene tiling screen and integration of results with ClinVar screen.
From: Mapping variant effects on anti-tumor hallmarks of primary human T cells with base-editing screens
a, sgRNA LFCs across both donors at Day 26 of the long-term proliferation arm of the 12-gene tiling screen are plotted. Dotted lines represent top and bottom 10% cutoffs of the distribution of negative control sgRNAs (empty window and silent only sgRNAs) for each donor. Selected sgRNAs, with predicted target gene and mutation, are shown. b, sgRNA LFCs as in a, with blue overlay filtered by target gene. c, Shared sgRNAs (n = 325) were identified between the ClinVar and 12-gene tiling screens and sgRNA LFCs from matched long-term proliferation arm timepoints (Day 28 of ClinVar Screen, Day 26 of 12-gene Tiling Screen) are plotted. For each screen, the average LFC of each sgRNA across both donors is plotted. Selected sgRNAs with shared enrichment/depletion patterns across donors and screens are annotated. Simple linear regression (panel c).
