Extended Data Fig. 7: Enrichment and structure-function relationship of variants promoting T cell proliferation.
From: Mapping variant effects on anti-tumor hallmarks of primary human T cells with base-editing screens
a, Lollipop plot showing LFC of sgRNAs targeting PIK3CD at Day 15 post-electroporation in the long-term proliferation arm of the ClinVar screen. sgRNAs are mapped to the targeted region of the canonical isoform of PIK3CD (p110δ) and functional domains of the protein are annotated. Selected variants and their predicted mutational consequences are annotated. b,c, Timecourse line graphs of LFC of sgRNAs targeting PIK3CD in both donors in the long-term proliferation arm of the ClinVar screen. d, Lollipop plot for sgRNAs targeting AKT1 at Day 35 post-electroporation in the long-term proliferation arm of the ClinVar screen, mapped to the canonical AKT1 isoform. e, Timecourse line graphs of LFC of sgRNAs targeting AKT1 in the long-term expansion arm of the ClinVar screen. f, Structure and position of mutations in AKT1. (right) Overall predicted structure of AKT1 (blue) and mutated residues (red). (left) Wild-type (WT) and mutated (mut) residues (red). D323G is predicted to localize next to L14 (dark blue). g, Lollipop plots for sgRNAs targeting LCK at day 26 post-electroporation in the long-term proliferation arm of the 12-gene tiling screen, mapped to the canonical LCK isoform. h, Structure and position of mutations in LCK. (top) Overall predicted structure of LCK (blue) and mutated residues (red). (bottom) Wild-type (WT) and mutated (mut) residues (red).
