Extended Data Fig. 1: Accompaniment to Fig. 1.

a. Left: Edited phage T2 genomes (%). With forward (blue) or reverse (purple) RT-DNA. Open circles are three biological replicates, closed circles are means. Right: Recombitron editing at site 143,279 (R) (±SD) versus a dRT control (unpaired, two-sided t-test, P = 0.77). b. Editing of wild-type and mutant T4 without modified cytosines, shown as in a (N = 3) (two-way ANOVA, effect of modified bases, P = 0.0061). c. Editing (%) of lambda and lambda_ΔcI in different host strains at site 14,070 (R). Open circles show 3 (wt in bSLS.114), 5 (ΔcI in bSLS.114), and 2 (ΔcI in bCF.5) biological replicates, closed circles show the mean (one-way ANOVA, effect of strain/phage, P = 0.2421). d. Editing (%) of lambda without induction of CspRecT at site 14,126, shown as in a (N = 3). e. Titer (PFU/mL) of phage lambda, T7, and T5 after propagation through host cells of different conditions, compared to amount of phage added to the culture, without recombitrons (open black circles), with uninduced recombitrons (open pink circles), with induced recombitrons (closed pink circles), and with induced recombitrons targeting a different phage (closed blue circles). Individual biological replicates are shown. f. Editing (%) of lambda, T7, and T5 from the induced, on-target recombitron condition in panel d, shown as in a (N = 3). g. Editing (%) of T7 with supplemental expression of lambda genes gam or beta, shown as in a (N = 3, one-way ANOVA P < 0.0001). h. Editing (%) of lamda with supplemental expression of lambda genes gam or beta, shown as in a (N = 3, one-way ANOVA P = 0.0904). i. Editing (%) of T7 with supplemental expression of E. coli SSB and lambda genes gam or beta, shown as in a (N = 3, one-way ANOVA P < 0.0001). j. Editing (%) of lambda with supplemental expression of E. coli SSB and lambda genes gam or beta, as in a (N = 3, one-way ANOVA P < 0.0001). k. Editing (%) of lambda at site 14126 (F) compared to editing with supplemental expression of T5 SSB, shown as in a (N = 3). l. Editing (%) of T7 at site 22872 (R) compared to editing with supplemental expression of T5 SSB, shown as in a (N = 3). m. Editing (%) of T5 at site 88634 (F) with supplemental expression of E. coli SSB, T7 SSB, or T5 SSB, shown as in a (N = 3). n. Editing (%) of phages from the basal collection, Bas46 (A19798T) and Bas47 (A6332G), that contain modified bases with the RT induced (+) or uninduced (-), as in f (N = 3).