Extended Data Fig. 2: Accompaniment to Fig. 2.
From: Continuous multiplexed phage genome editing using recombitrons
a. Rate of acquiring only the scanning edit in lambda when donors contain both scanning and central edits. (open circles are biological replicates, closed circles are the mean). b. PAGE analysis of retron RT-DNA in different E. coli strains. c. Editing (%) of lambda with editing cultures started at different initial multiplicities of infection (MOI), using MG1655 (Δexo1,ΔrecJ) as the editing host (one-way ANOVA P < 0.0001) (open circles are 3 biological replicates, closed circles are the mean). d. Editing (%) of lambda with editing cultures incubated at different temperatures, using MG1655 (Δexo1,ΔrecJ) as the editing host (unpaired, two-sided t-test P = 0.0013) (open circles are 3 biological replicates, closed circles are the mean).
