Visualizing RNA molecules in live cells remains a challenge, and existing methods require genetic manipulation or have limited resolution. Our study overcomes these limitations by using the programmable CRISPR–Csm tool to bind and track individual transcripts in their native state.
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References
Hwang, D. W., Maekiniemi, A., Singer, R. H. & Sato, H. Real-time single-molecule imaging of transcriptional regulatory networks in living cells. Nat. Rev. Genet. 25, 272–285 (2024). This review discusses the advantages and limitations of existing live-cell RNA imaging methods.
Colognori, D., Trinidad, M. & Doudna, J. A. Precise transcript targeting by CRISPR-Csm complexes. Nat. Biotechnol. 41, 1256–1264 (2023). This paper describes the use of CRISPR–Csm for RNA-guided ablation or imaging of specific transcripts in human cells.
Femino, A. M., Fay, F. S., Fogarty, K. & Singer, R. H. Visualization of single RNA transcripts in situ. Science 280, 585–590 (1998). This paper reports single-molecule visualization of endogenous RNA by fluorescence in situ hybridization.
Buxbaum, A. R., Haimovich, G. & Singer, R. H. In the right place at the right time: visualizing and understanding mRNA localization. Nat. Rev. Mol. Cell Biol. 16, 95–109 (2015). This review summarizes the applications of live-cell RNA imaging methods.
Wang, J. Y. & Doudna, J. A. CRISPR technology: a decade of genome editing is only the beginning. Science 379, eadd8643 (2023). A review that summarizes CRISPR systems and applications.
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This is a summary of: Xia, C. et al. Single-molecule live-cell RNA imaging with CRISPR–Csm. Nat. Biotechnol. https://doi.org/10.1038/s41587-024-02540-5 (2025).
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A ‘CRISPR’ way to visualize RNA in live cells. Nat Biotechnol (2025). https://doi.org/10.1038/s41587-025-02560-9
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DOI: https://doi.org/10.1038/s41587-025-02560-9
