Supplementary Figure 9: Lineage-specific gene expression in embryos cultured in vitro.
From: Dynamic epigenomic landscapes during early lineage specification in mouse embryos
a The embryos were collected at E4.0 and were cultured in vitro for 4 days. Epiblast-like and VE-like tissues were dissected from in vitro cultured (IVC) embryos. b Barcharts showing the expression of marker genes for epiblast, VE/endoderm, ectoderm, PS, and mesoderm for lineages isolated from in vivo (red) and in vitro cultured (IVC) embryos (blue). As some germ layer markers are also expressed at earlier stages including epiblast (in vivo), only those that are exclusively expressed during gastrulation were examined. c Hierarchical clustering analysis of RNA-seq data for tissues isolated from in vivo embryos and IVC embryos. d Barcharts showing the expression levels of Dnmts in tissues isolated from IVC embryos and in vivo embryos
