Supplementary Figure 3: ADCY3 expression in BHK cells subsequent to transient transfection with pcDNA3 alone (CON) or human ADCY3 constructs (wt, mut I–IVb).
From: Loss-of-function mutations in ADCY3 cause monogenic severe obesity
a, Representative blots with anti-ADCY3 antibody (top) and anti-HSP70 antibody (bottom) as loading control. Molecular weight standards (in kDa) are given to the right. b, Quantification of blots normalized for each experiment to values obtained for ADCY3 wt. n = 7–9. Means are represented as horizontal bars, s.d. as boxes, s.e.m. as whiskers and points represent individual data. c, Coomassie Blue staining of the PVDF membrane shown in a.
