Supplementary Figure 4: Immunofluorescence images of BHK cells transiently transfected with plasmids encoding Myc-tagged ADCY3 wild-type or mutants (I, II, III, IVb).
From: Loss-of-function mutations in ADCY3 cause monogenic severe obesity
ADCY3 expression was visualized by either anti-Myc or anti-ADCY3 antibody followed by Cy3-coupled secondary antibody. Nuclei were stained with Hoechst 33342.
