Supplementary Figure 1: EBS specifically binds methylated H3K4 and H3K27 marks.
From: EBS is a bivalent histone reader that regulates floral phase transition in Arabidopsis
a,c, Systematic profiling of histone binding preferences of full-length GST-EBS on a histone peptide microarray. A representative array image shows EBS binding to H3K4me3 (a) and H3K27me3 (c). b,d, Relative intensity of selective H3K4me3-containing (b) and H3K27me3-containing (d) peptide species. Relative signal intensity is calculated by normalizing each mean signal intensity at 635 nm for triplicate spots to the highest signal on the individual subarray, after subtracting background signals (derived from empty spots) for all spots. e, Relative intensity of EBS binding with different peptides. Peptide species containing the same PTMs are grouped together.
