Fig. 7: TdHMA3-B1a complements Cd- and Zn-sensitive mutant phenotypes of yeast.
From: Durum wheat genome highlights past domestication signatures and future improvement targets
a, Growth (OD600) of Cd-sensitive ycf1 yeast expressing empty vector (EV, p413TEF), YCF1, TdHMA3-B1a, TdHMA3-B1a-D411A and TdHMA3-B1b in the presence of 40 and 55 μM Cd. Plotted growth curves are means of three (D411A), five (YCF1, B1a, B1b), or six (EV) experiments ± 95% confidence intervals shown as shaded backgrounds. b, Growth (OD600) of Zn-sensitive zrc1cot1 yeast expressing empty vector (EV, p413TEF), ZRC1, TdHMA3-B1a, TdHMA3-B1a-D411A and TdHMA3-B1b in the presence of 125 and 225 μM Zn. Plotted growth curves are means of three (B1b) or five (EV, ZRC1, B1a, D411A) experiments ± 95% confidence intervals shown as shaded backgrounds. c, Localization of TdHMA3-B1a-GFP and TdHMA3-B1b-GFP expressed in ycf1 (differential interference contrast (DIC) and merged images provide spatial references). Scale bars, 2 μm. d, Cd accumulation in ycf1 and Zn accumulation in zrc1cot1 expressing TdHMA3-B1a and transport activity knockout, TdHMA3-B1a-D411A, after exposure to 5 μM Cd or 50 μM Zn for 4 h. Data are shown as means ± 95% confidence intervals for n = 4 independent cultures (circles). Experiments repeated with similar results. P values were calculated by two-tailed, unpaired t-tests (d.f. = 6). The dashed lines indicate the Zn concentrations of the inoculating yeast cultures.
