Supplementary Fig. 5: Neuroectoderm (NE) differentiation of MKK7 and JUN KO hESC lines. | Nature Genetics

Supplementary Fig. 5: Neuroectoderm (NE) differentiation of MKK7 and JUN KO hESC lines.

From: Genome-scale screens identify JNK–JUN signaling as a barrier for pluripotency exit and endoderm differentiation

Supplementary Fig. 5

a, NE differentiation schematic. The day when NE differentiation was initiated is designated as D0. Cells were examined on D4, 6, 8 and 10 of NE differentiation. b, Immunostaining of PAX6, SOX1 and OCT4 on D10 of NE differentiation from WT, MKK7 KO and JUN KO cell lines. Images are from one experiment. c, Representative flow cytometry dot plots of D10 NE cells stained for PAX6 (WT, MKK7 KO and JUN KO cell lines). d, Flow cytometry quantification of differentiation efficiency based on the percentage of PAX6+ NE cells from D4, D6, D8, D10 (WT, MKK7 KO and JUN KO cell lines). n = 3 independent experiments (D6-10), n = 2 independent experiments (D4). Error bars indicate SD. Statistical analysis was performed by unpaired two-tailed Student’s t test. NS, not significant (P ≥ 0.05).

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