Supplementary Figure 1: Dux zygotic CRISPR-Cas9 injection does not impair mouse pre-implantation development. | Nature Genetics

Supplementary Figure 1: Dux zygotic CRISPR-Cas9 injection does not impair mouse pre-implantation development.

From: Loss of DUX causes minor defects in zygotic genome activation and is compatible with mouse development

Supplementary Figure 1

a) Schematic view of the genotyping primer design (not drawn in scale). b) Blastocyst rate of embryos following Cas9/sgRNA injection at 1-cell stage. The concentration of Cas9, and sgRNA1/2 are 100, 50, 50 ng/µl, respectively. Numbers in the parentheses are the embryos analyzed for each group. Two independent zygotic CRIPSR-Cas9 injections were performed. BDF1 strain was used for this experiment. c) An agarose gel image illustrating the nested PCR-based genotyping assay of the F0 blastocysts. Genotyping results of 13 embryos are shown in this image. Black and red arrows point to the embryos with mono-allelic and bi-allelic deletion of the Dux cluster, respectively. A full scan of the gel can be found in Supplementary Figure 6. d) Summary of the Dux cluster genotyping results of F0 blastocysts (n = 36).

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