Supplementary Figure 3: Loss of DUX does not impair ovulation and pre-implantation development.

a) Number of ovulated oocytes and 1-cell embryos (IVF) collected from WT/Het and KO female mice. Each grey dot represents a single female mouse analyzed (n = 9 for each group). Female mice for both groups were subject to superovulation. For 1-cell embryos, oocytes from WT/Het and KO females were fertilized with WT and KO sperm, respectively. Measure of center and error bar indicate mean and SD, respectively. Two-tailed student’s t-test was used to compare the numbers of oocytes and 1-cell embryos between Het/WT and KO groups (oocyte p = 0.97; 1-cell p = 0.85). b) Percentage of IVF-derived embryos that reached the 2-cell (~ 28 hpi), 4-cell (~ 48 hpi), morula (~ 78 hpi), and blastocyst (~ 120 hpi) stages. The IVF experiments were performed four times (denoted as dots) and in total 235 (from 13 mice) and 223 (from 11 mice) embryos were analyzed for control and KO group, respectively. Measure of center and error bar indicate mean and SD, respectively. Fisher’s exact test (two-sided) was used to compare blastocyst stage embryos between the two groups (p = 0.45). c) Representative images of WT/Het and MZ-KO embryos at ~ 120 hpi. The IVF experiments were performed four times. Scale bar, 100 μm.