Supplementary Figure 5: The expression levels of the majority of known DUX targets were not affected by Dux MZ-KO in 2-cell embryos. | Nature Genetics

Supplementary Figure 5: The expression levels of the majority of known DUX targets were not affected by Dux MZ-KO in 2-cell embryos.

From: Loss of DUX causes minor defects in zygotic genome activation and is compatible with mouse development

Supplementary Figure 5

a) Scatter plot comparing the gene expression levels between 2C-like (MERVL::GFP-positive) and non-2C cells (MERVL::GFP-negative). Two RNA-seq replicates (Hendrickson P.G. et al., Nat Genet. 49, 941–945, 2017) were used for differential gene expression analyses. Genes expressed higher in 2C-like cells and higher in non-2C cells are colored with red and blue, respectively (FC > 2 and FDR < 0.05). b) Genome browser views illustrating the expression levels of known DUX targets in 2C-like cells and 2-cell embryos. HA-DUX ChIP-seq track and RNA-seq tracks of non-2C (2C-) and 2C-like (2C+) cells were obtained from a previous study (Hendrickson P.G. et al., Nat Genet. 49, 941–945, 2017). Only uniquely aligned reads were used to generate the ChIP-seq and RNA-seq tracks.

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