Supplementary Figure 7: Transformation of Yangmai158 with a ubiquitin promoter-driven HisR ORF
. a, Representative disease symptom 20 dai in the T1 progenies of three wheat transformants (#37, #38 and #39) and recipient cultivar Yangmai158 (Y158) inoculated with F. graminearum spores that include a strain carrying the GFP gene. The spike pictures in the bottom panel were merged from continuous photos taken under fluorescent light at 488-nm excitation wavelength using a fluorescence microscope (BX53, OLYMPUS, Japan). b, Expression of the transgenes in the wheat transformants. Experiments were repeated independently three times with similar results. M, DNA size standard in bp. c, Expression of HisR ORF in #37, #38 and #39 compared with expression in the WSB tissues without Fusarium infection (as described in Supplementary Fig. 2b). 150-bp paired reads were generated with an Illumina platform HiSeq X Ten System, (n = 2 biologically independent samples for transgenic plants). The total effective read per sample is at least 29 million. d, NDS of the T1 progenies from the three wheat transformants at 20 dai, with the box-and-whisker plots showing the medians (orange line), upper and lower quartiles (box edges), 1.5 × interquartile range (whiskers), and outliers (dots). The number of inoculated spikes (n) and the P values for two-tailed Student’s t-test for comparison of the transgenic segregants to the transgene-negative segregants are shown. In the progenies, the segregants with transgene showed significantly better resistance to FHB than the non-transgenic segregants.
