Supplementary Figure 9: RNAi-mediated His silencing in the susceptible line PH691
. a, Semiquantitative RT-PCR of His-RNAi in T0 transformants #7, #11 and #13. M, DNA size standard in bp. The experiments were repeated independently three times with similar results. b, His expression level in the three T0 transgenic plants relative to His expression in PH691 based on quantitative RT-PCR, detected with primers for all three His homoeologs. Values are represented as the means ± s.d. (n = 3 technical replicates). c, Disease symptoms 15 dai of the T0 transgenic plants and PH691. Only one spike was used in inoculation. In a-c, PH691 indicates transgene-negative plants from the transformation experiment. d, Disease symptoms 15 dai of PH691 together with the T2 transgenic plants derived from #7 and #11. e, f, NDS (e) and LDR (f) of the T2 transgenic and their transgene-negative siblings, with the box-and-whisker plots showing the medians (orange line), upper and lower quartiles (box edges), 1.5 × interquartile range (whiskers), and outliers (dots). The number of inoculated spikes (n) and the P values for two-tailed Student’s t-test for comparison of the transgenic plants to transgene-negative siblings in each family are shown.
